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odyssey infrared fluorescent scanner  (LI-COR)


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    LI-COR odyssey infrared fluorescent scanner
    Odyssey Infrared Fluorescent Scanner, supplied by LI-COR, used in various techniques. Bioz Stars score: 99/100, based on 7143 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/odyssey+fluorescence+scanner/Odyssey+FC+Imaging+System/pmc13130404-157-29-33
    Average 99 stars, based on 7143 article reviews
    odyssey infrared fluorescent scanner - by Bioz Stars, 2026-10
    99/100 stars

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    Related Articles

    other:

    Article Title: URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers.
    Article Snippet: Antibodies used are URI (1:1000, Proteintech, 11277-1-AP,), SREBP1 (1:200, Santa Cruz, sc-13551), SCD1 (1:1000, ABclonal, A16429), FASN (1:1000, ABclonal, A19050), FADS2 (1:1000, ABclonal, A10270), GPX4 (1:1000, ABclonal, A11243), GCLM (1:1000, ABclonal, A11444), GCLC (1:1000, ABclonal, A4499), SLC7A11 (1:1000, Proteintech, 26864-1-AP), ACSL4 (1:1000, Proteintech, 22401-1-AP), MDM2 (1:1000, ABclonal, A13327), Myc-Tag (1:1000, ABclonal, AE010), Flag-Tag (1:1000, ABclonal, AE005), His-Tag (1:1000, ABclonal, AE003), HA-Tag (1:1000, ABclonal, AE008), ACC (1:1000, CST, 3676), TRIM28 (1:1000, Proteintech, 15202-1-AP,), P53 (1:1000, Proteintech, 10442-1-AP), P21 (1:1000, Proteintech, 10355-1-AP), USP5 (1:1000, ABclonal, A4202), USP7 (1:1000, ABclonal, A13564), USP14 (1:1000, ABclonal, A19589), Actin (1:5000, Proteintech, 66009-1-ig), GAPDH (1:5000, ABclonal, AC001).

    Article Title: Intranuclear paraspeckle‐circular RNA TACC3 assembly forms RNA‐DNA hybrids to facilitate MASH‐related hepatocellular carcinoma growth in an m 6 A‐dependent manner
    Article Snippet: After blocking, the NC/PVDF membranes were incubated with primary antibodies (NONO/p54 nrb Rabbit mAb, 1:1000, #10162S, Cell Signaling Technology; METTL14 Rabbit polyclonal antibody, 1:1000, #26158‐1‐AP, Proteintech, Wuhan, Hubei, China; METTL3 Rabbit mAb, 1:1000, #86132T, Cell Signaling Technology; KIAA1429 Rabbit mAb, 1:1000, #88358T, Cell Signaling Technology; β‐actin Rabbit mAb, 1:1000, #4970T, Cell Signaling Technology) at 4°C overnight.

    Incubation:

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Article Title: N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes
    Article Snippet: .. Immunocomplexes were incubated with the fluorescein-conjugated secondary antibody and then detected using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE). ..

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500 μg of protein) were mixed with 2 μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with protein G magnetic bead (Invitrogen) at 4 °C for 2 h. IP beads were then washed with lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5 min for immunoblot analysis.

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500μg of protein) were mixed with 2μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with proteinGmagneticbead (Invitrogen) at4 °C for 2 h. IP beadswere then washedwith lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5min for immunoblot analysis.

    Imaging:

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Fluorescence:

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Article Title: N6-methyladenosine modification promotes hepatocarcinogenesis through circ-CDYL-enriched and EpCAM-positive liver tumor-initiating exosomes
    Article Snippet: .. Immunocomplexes were incubated with the fluorescein-conjugated secondary antibody and then detected using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE). ..

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500 μg of protein) were mixed with 2 μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with protein G magnetic bead (Invitrogen) at 4 °C for 2 h. IP beads were then washed with lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5 min for immunoblot analysis.

    Article Title: PTEN status determines therapeutic vulnerability to celastrol in cholangiocarcinoma.
    Article Snippet: Background: A balanced protein homeostasis network helps cholangiocarcinoma (CCA) maintain their oncogenic growth, and disrupting proteostasis therapeutically will induce proteotoxic stress.. Phosphatase and tensin homolog (PTEN) have been reported to be involved in proteostasis, and PTEN-associated pathways are commonly altered in CCA.. Celastrol, a triterpene from plants, exhibits cytotoxic effects in various types of cancer.

    Article Title: Inhibition of ACLY overcomes cancer immunotherapy resistance via polyunsaturated fatty acids peroxidation and cGAS-STING activation
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody at a dilution of 1:10,000 for 1 hour at room temperature, imaging of blots was performed using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). ..

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500μg of protein) were mixed with 2μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with proteinGmagneticbead (Invitrogen) at4 °C for 2 h. IP beadswere then washedwith lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5min for immunoblot analysis.

    Western Blot:

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500 μg of protein) were mixed with 2 μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with protein G magnetic bead (Invitrogen) at 4 °C for 2 h. IP beads were then washed with lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5 min for immunoblot analysis.

    Article Title: PTEN status determines therapeutic vulnerability to celastrol in cholangiocarcinoma.
    Article Snippet: Background: A balanced protein homeostasis network helps cholangiocarcinoma (CCA) maintain their oncogenic growth, and disrupting proteostasis therapeutically will induce proteotoxic stress.. Phosphatase and tensin homolog (PTEN) have been reported to be involved in proteostasis, and PTEN-associated pathways are commonly altered in CCA.. Celastrol, a triterpene from plants, exhibits cytotoxic effects in various types of cancer.

    Article Title: TET2-mediated tumor cGAS triggers endothelial STING activation to regulate vasculature remodeling and anti-tumor immunity in liver cancer.
    Article Snippet: .. After incubation with fluorescein-conjugated secondary antibody for 1 h at room temperature, the immunoblots were visualized using an Odyssey fluorescence scanner (Li-Cor, Lincoln, NE, USA). .. For co-IP, cells lysates (500μg of protein) were mixed with 2μg anti-Tet2 (GeneTex) overnight at 4 °C and then pulled down with proteinGmagneticbead (Invitrogen) at4 °C for 2 h. IP beadswere then washedwith lysis buffer three times, and heated in SDS sample loading buffer at 100 °C for 5min for immunoblot analysis.



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    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    LI-COR infrared fluorescence scanner
    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular <t>fluorescence</t> complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.
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    Image Search Results


    (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Journal: Cell reports

    Article Title: A shear stress-responsive pathway in monocytes drives cardiopulmonary bypass-induced inflammation via spectrin/RAF1/store-operated calcium entry

    doi: 10.1016/j.celrep.2025.116903

    Figure Lengend Snippet: (A) Schematic of the STIM1/ORAI1 complex using split Venus as a yellow signal reporter in a bimolecular fluorescence complementation (BiFC) assay. Yellow fluorescence indicates STIM1/ORAI1 interaction. The signal is higher after shear stress compared to the static condition in THP-1 cells. (B) Quantification of the Venus-positive BiFC STIM1/ORAI1 THP-1 cells under shear stress and static conditions. The shear stress condition shows 10.60%-±0.70% Venus-positive cells, while the static condition only has 1.80% ±0.20% Venus-positive cells. (C) STIM1-KD and ORAI1-KD cells have decreased shear stress-mediated IL8 expression as compared to control THP-1 cells, as quantified by qPCR. Replicates, n = 6. (D) SPTAN1-KD and RAF1-KD cells have decreased STIM1 and ORAI1 interaction under shear stress condition based on decreased split Venus STIM1 and ORAI1 signal. Replicates, n = 6. (E) Quantification of flow cytometry data, showing that STIM1-KD and ORAI1-KD cells have decreased shear-mediated cellular necrosis and increased cell survival. Replicates, n = 6. (F) Proposed mode of the cascade molecular reaction induced by shear stress. (G) FR180204 , an ERK inhibitor, decreased shear stress-mediated IL8 expression in CD14 + primary monocytes. (H) Quantification of flow cytometry data, displaying a decrease in shear stress-mediated necrotic cells following treatment with FR180204 in CD14 + primary monocytes.

    Article Snippet: Co-detection was performed using IRDye 700 and 800-labeled secondary antibodies (926–68070 and 926–32211, Li-Cor) under the Odyssey infrared laser fluorescence scanner (Li-Cor) and quantified using ImageJ.

    Techniques: Bimolecular Fluorescence Complementation Assay, Fluorescence, Shear, Expressing, Control, Flow Cytometry